Tips from the Bench

Protocols, recipes, troubleshooting and things I wish someone had told me at the bench.

Practical notes from hands-on lab work. Short when the answer is short; detailed when it needs to be.

Protocols

Step-by-step methods with the practical details that are easy to leave out of a methods section.

A practical manual IHC protocol for FFPE tissue using HRP-polymer detection and DAB, including the small details that matter at the bench.

Buffers, solutions, dilutions and calculations for everyday laboratory work.

No published tips in this category yet.

What to check when the experiment is not behaving as expected.

No published tips in this category yet.

Staining chemistry, slide processing and practical histology details from hands-on work.

Why hematoxylin needs bluing, why tap water works, and three simple bluing solutions you can prepare instead of leaving the tap running.

Handling tricks, setup choices and small observations collected through repeated lab work.

No published tips in this category yet.